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Most of the current homocysteine-lowering therapies are based on the supplementation of folic acid that can facilitate the remethylation of homocysteine [4]

(PubMed) Jacques PF, Bostom AG, Wilson PW, Rich S, Rosenberg IH, Selhub J

Lactic acid production from a whole slurry of acid-pretreated spent coffee grounds by engineered Saccharomyces cerevisiae

Western blot analysis After collecting and homogenizing hepatic tissue samples, we extracted the total protein from the supernatant, and subsequently, we measured the protein concentration based on the Lowry assay