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Current research methods include counting pups ultra-sound vocalizations during maternal separation, total locomotion and motor behaviors, activity trends in an unfamiliar environment, maze learning, and pain responsiveness [37,40]

Their white epididymal and brown interscapular adipose tissues were dissected, weighed, and frozen in liquid nitrogen for later RNA analysis

Analysis of the OPLS-DA scores plot revealed the model to account for more variance than an orthogonal data matrix of equal dimension (Figure 6A)

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