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In addition, by screening differentially expressed genes (such as endothelial NOS and inflammatory factors) through transcriptomics, validating key proteins (such as VEGF and ICAM-1) through proteomics, and analyzing small molecule metabolites (such as NO and oxidative stress markers) through metabolomics, a molecular network of endothelial injury can be jointly constructed [33]

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If your protocol isn't producing expected outcomes and you've ruled out dosage and administration errors, revisit your reconstitution technique