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In the present study, a 6-h application duration was chosen to ensure better compliance

Deglutamylation activities using -glutamyl- p -nitroanilide as the substrate were determined spectrophotometrically according to a previously described method (Orlowski and Meister, 1963), with slight modifications, as follows: the reaction mixture, which consisted of 0.0125 g l -1 yeast crude protein, 50 mM Tris-HCl (pH 8.0), 10 mM glycylglycine, and 1 mM -glutamyl- p -nitroanilide, was incubated for 6 h at 37C, and p -nitroaniline released from -glutamyl- p -nitroanilide was monitored at 412 nm

Product Format and Claims: Ensure the serum is marketed for "beard growth," "scalp care," or "hair density" with appropriate viscosity for facial application

Store at temperatures not exceeding 30C

Because AUC could not be extrapolated to infinity using regression analysis, it is difficult to assess the sufficiency of sampling times

To validate the reliability of the prediction model, permutation test ( n = 300) was calculated (Fig